- All projects should be briefly discussed with the Facility Manager before submitting any samples. Sample preparation prior to mass spectrometric analysis is crucial in obtaining the best qualitative results and should thus be carefully discussed.
- The ideal sample submission format, as well as some recommended procedures are presented here. Following these recommendations will ensure a smooth and rapid processing of your sample.
- Results will be sent back be e-mail in an electronic format report form. If additionnal or specific information is required (sequences, peak lists), please do not forget to mention it on the submission form.
- Complementary infomation can be obtained by combining different mass spectrometry analysis methods. Talk to us about what you would like to know about your sample. We will help you fill the different submission forms and interpret the output from the combined techniques.
- At this time, the facility is not able to accept any radiolabeled samples.
Please, make sure that you DO NOT submit any RADIOLABELED SAMPLES FOR ANALYSIS.
- We will strive to ensure rapid turn around of sample analyses.
Sample Submission Procedure
- Contact the facility manager to briefly discuss the project.
- Read the sample preparation guidelines carefully.
- Send the submission form(s) by e-mail or FAX together with additional helpful documents such as gel images, purification pipeline and conditions descriptions.
- You will be quickly contacted by a member of the facility to organize the sample preparation, shipment and reception. This person will be your contact person for any questions related to the processing and the analysis of your sample.
Ideally the sample amount should be above 2 pmol and free of any contaminant. Lower quantities can be detected but results will be sample dependent. The sample submission format for each analysis type should follow the rules described below in order to ensure the best qualitative result.
Non-fixing gel staining procedures are compatible with in gel digestion and extraction procedures. Coomassie staining is strongly recommended (R250 or colloidal G250). Silver stained gels can also be processed although this staining procedure is not the preferred one. In this case we recommend using non-fixing procedures (without glutaraldehyde or formaldehyde). For more detailed information see also our Protocols section .
Extreme caution should be taken when handling the gels to avoid contamination with keratins.
- Tie your hair back and avoid breathing on top of your sample.
- Always use powder-free gloves and clean scalpel or pipette tips when cutting gel bands or spots.
- Gels should be cut in the most strongly stained area on a pre-cleaned surface under water and ideally under a laminar flow hood.
- Gel bands should be “sliced” into small pieces (1 mm3) and collected into clean Eppendorf tubes in 1% acetic acid or 10% ethanol.
We recommend sending a negative control together with your sample.
- A good negative control for 1-D Gels is a piece of gel containing no protein cut at the same level as the band of interest. This “blank” sample will be processed in parallel to your sample to check for the presence of unexpected contaminants.
In solution samples:
- Concentrated samples in small volumes are preferable (10 to 20 μl);
- Do not forget to communicate the exact solution composition and pH; Salts should be avoided whenever possible.
- If not, an additional desalting step will probably be performed on your sample (C18 ZipTip).
- Detergents and additives such as polymers strongly interfere with the mass spectrometric analysis and should thus be avoided.
- The presence of organic solvents into the sample should be mentioned as well.
Freshly prepared samples will always result in better qualitative results. Avoid thus whenever possible to store your samples for long periods before submitting them to the mass spectrometry analysis.
It is strongly recommended to contact the facility manager before preparing the sample to discuss ideal sample conditioning.